Nile red: a selective fluorescent stain for intracellular lipid droplets.
نویسندگان
چکیده
منابع مشابه
Nile red: a selective fluorescent stain for intracellular lipid droplets
We report that the dye nile red, 9-diethylamino-5H-benzo[alpha]phenoxazine-5-one, is an excellent vital stain for the detection of intracellular lipid droplets by fluorescence microscopy and flow cytofluorometry. The specificity of the dye for lipid droplets was assessed on cultured aortic smooth muscle cells and on cultured peritoneal macrophages that were incubated with acetylated low density...
متن کاملSpectrofluorometric studies of the lipid probe, nile red.
We found that the dye nile red, 9-diethylamino-5H-benzo[alpha]phenoxazine-5-one, can be applied as a fluorescent vital stain for the detection of intracellular lipid droplets by fluorescence microscopy and flow cytofluorometry (J. Cell. Biol. 1985. 100: 965-973). To understand the selectivity of the staining, we examined the fluorescence properties of nile red in the presence of organic solvent...
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Microalgae are currently emerging as one of the most promising alternative sources for the next generation of food, feed, cosmetics and renewable energy in the form of biofuel. Microalgae constitute a diverse group of microorganisms with advantages like fast and efficient growth. In addition, they do not compete for arable land and offer very high lipid yield potential. Major challenges for the...
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A highly stable, long wavelength polarity sensitive probe, 8-nitrophenyl-3,5-dipiperidine-4,4-difluoro-4-bora-3a,4a-diaza-s-indacene (NPBDP), was developed for living cell imaging of intracellular lipid droplets by fluorescence microscopy.
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Lipid droplets (LDs) are unique intracellular organelles that are mainly constituted by neutral lipids (triglycerides, sterol esters). As such they serve as the main site of energy storage in the cell and they are akin to oil emulsions in water. To prevent the direct exposure of the hydrophobic neutral lipids to the aqueous environment of the cytosol, LDs are surrounded by a monolayer of phosph...
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ژورنال
عنوان ژورنال: The Journal of Cell Biology
سال: 1985
ISSN: 0021-9525,1540-8140
DOI: 10.1083/jcb.100.3.965